Recombinant Protein - LRRK2tide (Nictide)

Name: LRRK2tide (Nictide)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU17157
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Calculated molecular mass:
Mono-Isotopic Mass: 29, 555.14 daltons
Average Mass: 29, 574.33 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
8.19
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids R1 – R21 (end) of the LRRK2 substrate peptide. Residue R232 of fusion protein is equivalent to R1 of the LRRK2 substrate peptide The GST tag is located at residues 1 – 220.
Protease Cleavage:
Prescission site (LEVLFQGP) at residues 221 – 228
Cloning Sites:
BamH1 and Not1 site of pGex6P-1

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