Recombinant Protein - MAPKAP-K3 (2 - 382)

Name: MAPKAP-K3 (2 - 382)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
DU929
Accession:
NM_004635
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Sepharose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2-mercaptoethanol, 0.1mM EGTA, 10 mM MgAc
Enzymatic Substrate:
KKLNRTLSVA Final concentration: 30 μM
Calculated molecular mass:
Mono-Isotopic Mass: 69, 635.1 daltons
Average Mass: 69, 679.96 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 50 % glycerol, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 0.2 mM PMSF, 1 mM Benzamidine
Storage Temperature:
-20 °C
Theoretical pI:
6.34
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids D2 – Q382 (end) of human MAPKAP-K3. Residue D232 of fusion protein is equilivalent to D2 of the native enzyme. The GST tag is located at residues 1- 220.
Protease Cleavage:
PreScission (LEVLFQGPL) at residues 221 – 229
Cloning Sites:
BamH1 and EcoR1 of pGEX6P-1

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