Recombinant Protein - MAPKAP-K1b (RSK2) (2 - 740)

Name: MAPKAP-K1b (RSK2) (2 - 740)
Price per aliquot (100µg): £110.00
Long Name:
ribosomal protein S6 kinase A3
Synonyms:
RPS6KA3, ribosomal protein S6 kinase, 90kD, polypeptide 3, HU-3
Catalog Number (DU Number):
DU1846
Accession:
NM_004586
Expression:
baculovirus
Tag and Terminus:
N-Term
6His
Uncleaved
Purification Method:
Ni2+-NTA agarose
Enzymatic Assay Format:
Standard filter binding assay
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Enzymatic Substrate:
Long S6 [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK] Final concentration: 30 μM
Calculated molecular mass:
Mono-Isotopic Mass: 84, 678.34 daltons
Average Mass: 84, 731.96 daltons
Purity:
>95 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF.
Storage Temperature:
-70 °C
Theoretical PI:
6.52
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids P2 – L740 (end) of human RSK2. Residue P11 of the fusion protein is equilivalent to P2 of the native enzyme. The His(6) tag is located at residues 3 – 8. The following amino acid substitution is present: V – G, where V45 of the native sequence is G54 of the fusion protein.
Cloning Sites:
Nde1 and Xho1 sites of modified pFastBAC 1.

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