Name: MAPKAP-K1a (RSK1) (1 - 735)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
Enzymatic Assay Format:
Standard filter binding assay
50 mM Tris-HCl pH 7.5, 0.1 % 2−mercaptoethanol, 0.1 mM EGTA, 10 mM MgAc
Long S6 [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK] Final concentration: 30 μM
Calculated molecular mass:
Mono-Isotopic Mass: 88, 284.23 daltons
Average Mass: 88, 284.23 daltons
50 mM Tris-HCl pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF.
Amino acids M1 – L735 (end) of rat RSK1.
Residue M47 of the fusion protein is equivalent to M1 of the native enzyme. The His(6) tag is located at residues 5 – 10 and the HA tag is located at residues 36 – 44.
The following amino acid ubstitutions are present:
E – G, where E551 of the native sequence is G597 of the fusion protein. S – N, where S637 of the native sequence is N683 of the fusion protein. G – A, where G697 of the native sequence is A743 of the fusion protein. The additional glutamine, Q157 (native sequence) reported in Dalby et al. (1998) J.Biol.Chem. 273, 1496-1998, has been removed from the fusion protein.
rTEV (ENLYFQG) residues 18 - 24
Not1 site in pFastBAC HTb