Polyclonal Antibody - Raptor

Name: Raptor
Price per aliquot (100µg): £110.00
Full Name:
Raptor (1 - 20)
Long Name Text:
Regulatory Associated Protein Of MTOR
Immuno Sequence:
MESEMLQSPLLGLGEEDEAD [residues 1 - 20 of human]
Phosphopeptide:
MESEMLQSPLLGLGEEDEAD [residues 1 - 20 of human]
Sheep Number:
S260D
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against recombinant protein and then depleted against GST.
Gel Image:

Raptor was immunoprecipitated from HEK 293 cellular extracts (A -3 mg or B – 1 mg) using 2 μg of anti-Raptor . Immunoprecipitates were subjected to SDS-Page analysis before being transferred to nitrocellulose membrane and immunoblotted with anti-Raptor at 1 μg/ml. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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